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Library of targeted real time PCR primer sets (10 u M, 40 ul)Perform up to 200 PCR Arrays! (Based on a 10 ul reaction volume)Flexibility to design one's own experiments Microplate containing 88 targeted plus 8 housekeeping gene primer sets (20ul per well, 10u M concentration)Note: Primer sequences unavailable for primer libraries If you would like to request a specific library for a pathway or organism not listed, please let us know: [email protected] goal is to provide a cost-effective approach to validate NGS or microarray data and quantitate gene expression using real time PCR assays Perform up to 1000 assays Detailed information on optimal reaction conditions provided Primer sequences provided!

Overview - Quantitative "Real Time" PCRQuantitative real time PCR has revolutionized our ability to measure nucleic acid concentrations and is an important step for the validation of expression data generated by microarray analysis and other genomics techniques.

Loor Copyright © 2014 Roberta Lane de Oliveira Silva et al.

A variety of applications have emerged as a result of the discovery of real time PCR.

These include 1) validation of gene expression data obtained by microarray analysis or next generation sequencing (NGS), 2) measurement of DNA copy number, 3) detection and quantitation of viral particles and potentially lethal microorganisms, 4) mutation/SNP analysis, and 5) mi RNA expression.

Ideally, they should be neither induced nor repressed under different experimental conditions.

To date, few reference genes have been adequately studied for sugarcane (Saccharum spp.) using statistical approaches.

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