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Non-Purdue users, may purchase copies of theses and dissertations from Pro Quest or talk to your librarian about borrowing a copy through Interlibrary Loan.(Some titles may also be available free of charge in our Open Access Theses and Dissertations Series, so please check there first.) Access to abstracts is unrestricted. Poster #: 1 Campus: CSU Northridge Poster Category: Agriculture/Biofuels/Environment Keywords: Arabidopsis thaliana, Genetic engineering, Weather pollution Project Title: Overexpression of an aminocyclopropane-1-carboxylic acid synthase gene in Arabidopsis thaliana and its effect on removing environmental pollutants Author List: Ramadoss, Niveditha; Graduate, Biology, California State University, Northridge, Presenting Author Gupta, Dinesh; Washington University, St.Louis Guenther, Alex; University of California, Irvine Basu, Chhandak; Biology, California State University, Northridge Abstract: Rise in air pollution has led to increasing cancer, several respiratory and cardiac illnesses in humans.To achieve this goal we genetically transformed Arabidopsis thaliana with an ACS (Aminocyclopropane-1-carboxylic acid synthase) gene.
Links to log in to the proxy server directly below the download button of each thesis or dissertation page. Therefore, we hypothesize that ACS gene might play an important role in poplar plants in mitigating stresses resulting from pollutant exposure. The homolog of poplar ACC synthase gene was PCR amplified from Arabidopsis thaliana and cloned into p BINmgfp5-er vector, featuring a constitutive cauliflower mosaic virus promoter removing the native jellyfish green fluorescent (GFP) gene. The Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines propose that the abbreviation q PCR be used for quantitative real-time PCR and that RT-q PCR be used for reverse transcription–q PCR. Cells in all organisms regulate gene expression by turnover of gene transcripts (single stranded RNA): The amount of an expressed gene in a cell can be measured by the number of copies of an RNA transcript of that gene present in a sample.
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